Journal: Frontiers in Cardiovascular Medicine
Article Title: Lipoprotein(a) Induces Vesicular Cardiovascular Calcification Revealed With Single-Extracellular Vesicle Analysis
doi: 10.3389/fcvm.2022.778919
Figure Lengend Snippet: Lp(a) enriched annexins in human SMC and VIC EVs. Western blots with equally loaded (protein abundance) human (A) SMCs total cell lysate and SMC EVs lysate and (B) VICs total cell lysate and VIC EVs lysate for annexins A1 (ANXA1), A2 (ANXA2), and A6 (ANXA6). Example blots shown, with CD29 and GAPDH used as loading controls for quantification; n = 4 donors; analyzed by ANOVA, * P < 0.05, ** < 0.01, **** < 0.0001, error bars = STDEV. Blots were stripped and reprobed for ANXA1, ANXA2 and GAPDH antibodies.
Article Snippet: Primary human coronary artery smooth muscle cells (SMCs) were obtained from Promocell (Heidelberg, Germany) and expanded in Promocell SMC Growth Medium 2 with Promocell SMC media supplement containing epidermal growth factor (0.5 ng/mL), insulin (5 μg/mL), basic fibroblast growth factor-β (2 ng/mL), and 5% fetal bovine serum.
Techniques: Western Blot